Physical, chemical, and cell toxicity properties of mature/aged particulate matter (PM) trapped in a diesel particulate filter (DPF) along with the results from freshly produced PM of a diesel engine.

The lifetime and efficiency of diesel particulate filters (DPFs) strongly depend on the proper and periodic cleaning and servicing. Unfortunately, in some cases, inappropriate methods are applied to clean the DPFs, e.g., using air compressors without proper disposal procedures which can have negative impacts on human health, the environment, and DPF's efficiency. However, there is no information available about the properties of this kind of PM. This research is therefore presented to explore the physicochemical and toxicity properties of aged PM trapped in a DPF (using compressed air for PM sampling) employing STEM, SEM, EDS, Organic Carbon Analyzer, TGA/DSC, and Raman Spectrometer for investigating the physicochemical properties, and assays of cell viability, cellular reactive oxygen species (ROS), interleukin-6, and tumor necrosis factor-alpha (TNF-α) for investigating the toxicity properties. Also, analyses from fresh PM samples from the diesel vehicle at two engine speeds are presented. It is found that at a certain/fixed PM number/mass for all three samples tested, the PM from DPF compared with the fresh PM can have both positive (particularly having the lowest water-soluble total carbon ratio) and negative impacts on human health (particularly having the highest cell death rate of 13.4%, ROS, and TNF-α) and the environment.

Physicochemical and cell toxicity properties of particulate matter (PM) from a diesel vehicle fueled with diesel, spent coffee ground biodiesel, and ethanol.

The literature shows that information about the physical, chemical, and cell toxicity properties of particulate matter (PM) from diesel vehicles is not rich as the existence of a remarkable number of studies about the combustion, performance, and emissions of diesel vehicles using renewable liquid fuels, particularly biodiesels and alcohols. Also, the PM analyses from combustion of spent coffee ground biodiesel have not been comprehensively explored. Therefore, this research is presented. Pure diesel, 90% diesel + 10% biodiesel, and 90% diesel + 9% ethanol + 1% biodiesel, volume bases, were tested under a fast idle condition. STEM, SEM, EDS, Organic Carbon Analyzer, TGA/DSC, and Raman Spectrometer were employed for investigating the PM physical and chemical properties, and assays of cell viability, cellular reactive oxygen species, interleukin-6, and tumor necrosis factor-alpha were examined for investigating the PM cell toxicity properties. It is found that the application of both biodiesel and ethanol has the potential to change the PM properties, while the impact of ethanol is more than biodiesel on the changes. Regarding the important aspects, biodiesel can be effective for better human health (due to a decrease in cell death (-60.8%)) as well as good diesel particulate filter efficiency (due to lower activation energy (-7.6%) and frequency factor (-83.2%)). However, despite a higher impact of ethanol on the reductions in activation energy (-24.8%) and frequency factor (-99.0%), this fuel causes an increase in cell death (84.1%). Therefore, biodiesel can be an appropriate fuel to have a positive impact on human health, the environment, and emissions catalysts performance, simultaneously.

Why is the world not yet ready to use alternative fuel vehicles?

Despite the improvement in technologies for the production of alternative fuels (AFs), and the needs for using more AFs for motor vehicles for the reductions in air pollution and greenhouse gases, the number of alternative fuel vehicles (AFVs) in the global transportation sector has not been increasing significantly (there are even small drops for adapting some AFs through the projections) in recent years and even in the near future with projections to 2050. And gasoline and diesel fuels will remain as the main energy sources for motor vehicles. After reviewing the latest advantages and disadvantages of AFVs, including flexible-fuel, gas, electric, hybrid electric, and fuel cell electric vehicles, it is found that the higher price of AFVs, compared to that of gasoline and diesel vehicles, might be one of the main barriers for their wider adoption. But on the other hand, there is the "chicken and egg" problem. Because people mostly do not select AFVs due to their higher price and sometimes their less infrastructure availability compared to those of gasoline and diesel vehicles, however, governments and AFVs manufacturers claim that the insignificant demand volume and less interest by people to purchase them, is one of the main reasons for a higher price and less infrastructure availability of AFVs. So, what should we do for adopting AFVs? This review shows that there are two very important and fundamental points that might cause a rise in the demand and usage of AFVs, rather than waiting for the reduction in AFVs prices. Those points are car salespeople's and people's knowledge about AFVs and the environmental issues, and their encouragement to accept and use AFVs. Although the AFVs are available on the market for many years, many people around the world have no/less/old/wrong knowledge about the current AFVs. Thus, most of these people reject these vehicles for usage, even when their important parameters such as purchase price, operating cost, driving range, and fuel availability be the same (or close) as those of gasoline or diesel vehicles. Detailed information, examples, and recommendations to the increases in people's knowledge and encouragement are presented in this review.

Author Correction: Drug screening of cancer cell lines and human primary tumors using droplet microfluidics.

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

Drug screening of cancer cell lines and human primary tumors using droplet microfluidics.

Precision Medicine in Oncology requires tailoring of therapeutic strategies to individual cancer patients. Due to the limited quantity of tumor samples, this proves to be difficult, especially for early stage cancer patients whose tumors are small. In this study, we exploited a 2.4 × 2.4 centimeters polydimethylsiloxane (PDMS) based microfluidic chip which employed droplet microfluidics to conduct drug screens against suspended and adherent cancer cell lines, as well as cells dissociated from primary tumor of human patients. Single cells were dispersed in aqueous droplets and imaged within 24 hours of drug treatment to assess cell viability by ethidium homodimer 1 staining. Our results showed that 5 conditions could be screened for every 80,000 cells in one channel on our chip under current circumstances. Additionally, screening conditions have been adapted to both suspended and adherent cancer cells, giving versatility to potentially all types of cancers. Hence, this study provides a powerful tool for rapid, low-input drug screening of primary cancers within 24 hours after tumor resection from cancer patients. This paves the way for further technological advancement to cutting down sample size and increasing drug screening throughput in advent to personalized cancer therapy.